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I work on a really important organism called Phytophthora infestans, which is what caused the Irish Potato Famine many years ago. It’s still a big problem for farmers today and makes food more expensive in the supermarkets! It completely devastates the plants it infects and spreads very quickly. Its name ‘Phytophthora‘ is even Greek for ‘Plant Destroyer’.
What I’m trying to understand is how Phytophthora is able to stop the plant’s natural immune system from defending itself from attack. In the laboratory I work in, one of the most important tools we use is X-Ray crystallography to understand the structure of important molecules. We believe that by studying the structure of molecules that Phytophthora produces, and by studying the structure of the molecules in the plant they are targeting, we will be able to get a better understanding of how this devastating disease is able to cause us so many problems.

A day in the life of Ben Hall:

I get up somewhere between 07.30 and 08.00. It only takes me five minutes to cycle to work so I don’t need to be up very early. I have breakfast and a cup of coffee (this is very important, I’m more or less fuelled by caffeine) then shower before setting off for work.

I get in for about 09.00 and one of the first things I do is check our internal website for any interesting seminars that day. One of the real perks of working at a world leading institute is that on any day of the week you can go and hear someone talking about truly great science. I’m always astonished at the work people are doing around the site. If I see something interesting, I’ll do my best to make time to go and see it.

The next thing I’ll do is have a look at my to do list. I typically write one on Sunday evening for the week ahead. I do my best to stick to it. However, science can be cruel and plans can go out of the window. The list helps me decide what needs to be done.

Ok, my list tells me that today I’ve planned to check if two proteins I’ve taken out of plant leaf tissue interact and also that I need to transfer some genes I’ve altered into more plants. So before lunch I know I need to prepare my plant-infecting bacteria for the afternoon. To do this I’ll bathe them in a mixture of chemicals which make them more likely to transfer their genes into the plants own DNA. For now I can forget about this and leave them on my work bench for a few hours.

Now onto the proteins I’ve extracted from the plant tissue. The previous day I’ll have separated out all of the proteins that are in the plant cells and attached them to a thin membrane. I’ll collect this membrane in the morning and start bathing it in molecules which stick to the proteins I’m interested in and give off a signal I can detect, such as fluoresence. I’ll use several different ‘sticky’ molecules in order to figure out whether or not the proteins are interacting with each other. This takes up pretty much a full day.

At about 12.30 I go for lunch. I usually sit with the other PhD students who all work in different labs to me. It’s good to be able to catch up with what they’re doing at work and to talk about any problems that you’re having. We all come from different backgrounds but get along really well.

After lunch my plant-infecting bacteria should be ready to go. So I take them down to the glasshouses and use a syringe without the needle attached to force them into the plant leaves. This can take 10 minutes or several hours depending on how many plants I need to infect. While I’m down there, I may need to collect leaves that I’d previously infected. To do this, I simply cut them off with scissors before freezing them in liquid nitrogen, which is -196 degrees centigrade!

When I get back from the greenhouses I’ll keep going with my ‘sticky’ molecules and membranes. At some point in the afternoon I’ll grab a coffee, check my e-mails and maybe have a quick look at Twitter.

For the rest of the day I could be preparing bacteria for tomorrow or the day after tomorrow. Purifying DNA from bacteria and shuffling it into new bacteria. Taking photos of plants that are reacting in an interesting way to the genes I’ve inserted or any number of other things like reading about the work other scientists have done, writing about my own work or discussing plans for experiments with my boss.

I generally leave work at some point between 17.30 and 20.00 and cycle straight to the gym or Rugby training. When I’m home I sometimes have more work to do but try my best to avoid taking work home. I’ll relax by reading (currently enjoying Sherlock Holmes), learning German or going to the pub with friends.